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詳細介紹
準備最終體積 80% ~ 90% 的配制用水（超純水、注射用水等）。稱取與配制量相對應的粉末培養基（13.5 g/L）。溫和攪拌配制用水，邊攪拌邊加入稱好的粉末培養基，持續攪拌至溶解。稱取所需量的，添加到培養基中，持續攪拌至溶解。測量培養基的 pH 值，若與目標值不同，則使用 1 N 的鹽酸或 1 N 的水溶液調整 pH 值。加入配制用水至最終容量，攪拌數分鐘使其均勻混合。此時若過度攪拌可能會導致 pH 值升高。使用孔徑為 0.1 μm 或 0.22 μm 的濾膜進行過濾，無菌分裝至滅菌容器中。分裝后的培養基請保存在 2 ~ 8°C 的陰涼避光處。
該粉末培養基需要密封保存，并放置在陰涼避光處（2-10°C）。
生產之后一年。
每 13.5 g (1 L)
Sodium chloride................................................ 6,400 mg
Potassium Chloride............................................... 400 mg
Calcium Chloride (anhydrate)................................... 200 mg
Magnesium sulfate (Anhydrate).................................. 97.7 mg
Sodium Dihydrogen Phosphate (Dihydrate)..................... 125 mg
Iron(III)Nitrate (Enneahydrate)................................. 0.1 mg
Glucose............................................................. 4,500 mg
Sodium Pyruvate................................................... 110 mg
L-Arginine Hydrochloride....................................... 84 mg
L-Cystine Dihydrochloride....................................... 62.6 mg
Glycine............................................................. 30 mg
L-Histidine Hydrochloride Hydrate............................. 42 mg
L-Isoleucine....................................................... 104.8 mg
L-Leucine.......................................................... 104.8 mg
L-Lysine Hydrochloride.......................................... 146.2 mg
L-Methionine....................................................... 30 mg
L-Phenylalanine.................................................. 66 mg
L-Serine............................................................ 42 mg
L-Threonine....................................................... 95.2 mg
L-Tryptophan...................................................... 16 mg
L-Tyrosine Disodium............................................. 89.5 mg
L-Valine........................................................... 93.6 mg
Choline Tartrate.................................................. 7.2 mg
Folic Acid.......................................................... 4 mg
Nicotinamide..................................................... 4 mg
Calcium Pantothenate........................................... 4 mg
Pyridoxal Hydrochloride........................................ 4 mg
Riboflavin......................................................... 0.4 mg
Thiamine Hydrochloride........................................ 4 mg
Myo-nositol...................................................... 7.2 mg
Phenol Red......................................................... 15 mg
L-Glutamine....................................................... 584 mg
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